Rate-Limiting Pyrophosphate Release by HIV Reverse Transcriptase and its Effect on Enzyme Specificity
نویسندگان
چکیده
منابع مشابه
Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.
Sodium pyrophosphate inhibits synthesis of anticomplementary DNA during a reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) catalyzed reaction. In the presence of pyrophosphate, the complementary DNA remains stably complexed to the RNA template. In the absence of pyrophosphate, the DNA. RNA hybrid template is degraded and anticomplementary DNA is synthesized. High conc...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.165